DNA extraction from roots of xoconostle

Authors

  • David Montiel 1Departamento de Producción Agrícola y Animal. Universidad Autónoma Metropolitana-Xochimilco.A.P. 23-181, Mexico, D.F., 04960, México.
  • Ernestina Valadez Moctezuma 2Departamento de Fitotecnia. Universidad Autónoma Chapingo. Chapingo, Estado de México. 56230, México.
  • Guadalupe Palomino 3Instituto de Biología. Universidad Nacional Autónoma de México. Apdo. Postal 70–614, México D.F. 04510, México.
  • Mario A. Bermúdez 4Departamento de Biotecnología. CINVESTAV. A.P. 14-740. México, D.F. 07360, México.
  • Francisco J. Fernández 5Departamento de Biotecnología. Universidad Autónoma Metropolitana-Iztapalapa. A.P. 55-535, México, D.F., 09340, México.

DOI:

https://doi.org/10.56890/jpacd.v14i.82

Keywords:

Contaminating, polysaccharides, PCR

Abstract

High contents of polysaccharides present in cactus of the genus Opuntia make it difficult to obtain optimal quality DNA for genotyping analysis. In this work, DNA was extracted from the young roots of xoconostle (Opuntia matudae Scheinvar). The quality of the DNA was assessed by random amplification with PCR using RAPD. The extraction method will help to solve technical problems that are commonly reported by different researchers for this group of plants, and it will lead to more efficient handling of samples for DNA analysis. The use of small samples of young roots as an alternative to cladodes, which are normally used for genetic or genomic analysis, was appropriate because high concentrations of DNA with optimum quality were obtained, and this DNA contained low concentrations of the polysaccharides characteristic of this group of cacti. The amplified fragments obtained from the different plants were clear and reproducible.

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Published

10-07-2012

Issue

Section

Scientific Papers