Establishment and Transformation of Callus and Cell Suspension Cultures of the Prickly-Pear (Opuntia ficus-indica)

Autores/as

  • R. M. Llamoca-Zárate
  • F. A. P. Campos
  • J. Landsmann

DOI:

https://doi.org/10.56890/jpacd.v3i.140

Palabras clave:

Cell suspension, friable callus, Opuntia ficus indica, particle gun

Resumen

Friable callus cultures were initiated from cotyledons and hypocotils of Opuntia ficus-indica.
Propagation and growth of calli were observed on MS medium supplemented with 2,4-D, Pickloram
and Casein. Cell transformation was achieved by particle bombardment. Kanamycin resistance,
GUS expression, and PCR analyses indicated successful integration of foreign DNA.

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Journal of the Professional Association for Cactus Development
Editora: 
Professional Association for Cactus Development

Descargas

Publicado

04/18/1998 — Actualizado el 06/20/2020

Número

Vol. 3 (1998)

Sección

Scientific Papers